Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for alchemy for PCR, is a technique to exponentially amplify in vitro a diminutive quantity of a specific nucleotide sequence in the presence of model sequence, two oligonucleotide primers that hybridize to opposite strands and flank the region of interest in the target DNA, a thermostable (taq) DNA polymerase. The activity is cycled involving model denaturation, primer annealing, and the extension of the annealed primers by DNA polymerase until enough copies are made for boost analysis.